Rabies is an infectious disease that causes fatal encephalitis in humans and animals. For the protection from the disease, Novartis Vaccines and Diagnostics in Marburg produces the vaccine Rabipur®. This is a vaccine against the Rabies virus which is produced on primary chicken embryo cells. Within this master thesis a characterization of the cells was realized using particularly micro array and mass spectrometry techniques, which were also helpful in investigations concerning a new, not fully understood phenomenon. Further researches concerning the characterization were performed with flow cytometry and the enzyme linked immunosorbent assay, which were difficult to perform due to absence of avian antibodies and low homology between mammalian and avian proteins.
Studiengang / zentr. Einrichtung:
Biotechnologie / Biopharmazeutische Technologie Master